Planta Med 2012; 78 - PI191
DOI: 10.1055/s-0032-1320879

Anti-Mycobacterium tuberculosis activity of extracts with a new benzoic acid derivative from Piper diospyrifolium by supercritical carbon dioxide

RBL Scodro 1, SC Espelho 1, CTA Pires 3, VS Carrara 1, L Cardozo-Filho 3, VLD Siqueira 1, RF Cardoso 2, DAG Cortez 1
  • 1Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá (UEM), Paraná, Brazil
  • 2Programa de Pós-graduação em Biociências Aplicadas a Farmácia, (UEM), Paraná, Brazil
  • 3Departamento de Engenharia Química, (UEM), Paraná, Brazil

Piper diospyrifolium leaves extracts obtained by supercritical fluid extraction (SFE-CO2), a new benzoic acid derivative and their methylated derivative were evaluated against Mycobacterium tuberculosis. The SFE-CO2 was performed at temperatures ranging from 20°C to 40°C and pressure from 140 to 250bar. The SFE-CO2 extracts was purified by chromatography methods and a new compound 4-methoxy-3-[(1E)-3-methylbuta-1,3-dien-1-yl]-5-(3-methylbut-2-en-1-yl) benzoic acid was determined by spectroscopic methods and the methylated derivative was prepared. Anti-M. tuberculosis activity was carried out using resazurin microtiter assay plate (REMA)1. The cytotoxicity assay was carried out in macrophages J774G8 by sulforrhodamine B colorimetric assay2. Benzoic acid derivative, their methylated derivative and the SFE-CO2 extracts exhibited activity in M. tuberculosis H37Rv with MIC values of 125µg/mL. The cytotoxicity results showed selectivity index range from 0.6 to 1.0. Additional studies using benzoic acid derivative should be conducted for a better evaluation of anti-mycobacterial activity of these compounds.