Antiproliferative activity and ESI-MS/MS chemical profile of the crude extract and fractions from of Campomanesia adamantium

Leaves of Campomanesia adamantium were collected in Curitiba-PR, Brazil, dried, pulverized and subjected to maceration process with ethanol. The crude extract was fractionated by liquid-liquid extraction with hexane, dichloromethane and butanol. Samples were analyzed by direct insertion mass spectrometry with electrospray ionization (ESI-MS). The ion corresponding to compounds of interest were selected and submitted to collision induced dissociation. The compounds were identified by comparison of their ESI-MS/MS fragmentation spectra with that of standards. Antiproliferative activity was evaluated for a panel of human tumor cells, including U251, MCF7, NCI-H460, OVCAR-03, HT-29, 786–0, NCI-ADR/RES and non- cancer cell HaCat by sulforhodamine B assay. Samples were showed antiproliferative activities against some cell lines with GI 50 (concentration able to inhibit 50% growth) between 1.19µg/mL and 112.48µg/mL. In ESI-MS/MS analyses it was possible to identify gallic acid, 2', 4'-dihydroxy-6'-methoxychalcone, 2', 4'-dihydroxy-5'-methyl-6'-methoxychalcone, quercetin, quercitrin and isoquercitrin. Those compounds may be responsible for the antiproliferative activities from crude extraction and fraction.