stimulation of cellular proliferation of human fibroblasts and human keratinocytes cell by Eclipta prostrata and Litsea glutinosa extracts

Stimulation of cellular proliferation is central mechanism within the complex physiology of wound healing. This study aimed to investigate the influence of extracts (EtOH 95%) from the aerial parts from Eclipta prostrata Linn. (Asteraceae) and of an aqueous extract from Litsea glutinosa C.B.Robinson (Lauraceae) leaves on the proliferation of primary human dermal fibroblasts and immortalized human keratinocyte cell line (HaCaT). Both plants are extensively used within Thai traditional medicine for wound healing.

Results indicated that extracts from E. prostrata (50µg/mL) stimulated fibroblast cell proliferation in a dose-dependent manner to 196% (p<0.01). Also keratinocyte proliferation was stimulated at 1µg/mL to 139% (p<0.01).

L. glutinosa leaves are rich of polysaccharide. Fractionation of an aqueous extract yielded the high molecular raw polysaccharides (RPS) and the low molecular weight fractions (LMW). RPS was fractionated on DEAE-Sephacel ion exchange stationary phase. The neutral fraction exhibited 153% proliferation (p<0.01) in HaCaT cell at concentration of 100µg/mL but showed no significantly effects against dermal fibroblasts. The LMW fraction stimulated fibroblast proliferation to 256% (p<0.01) at concentration of 10µg/mL, and increased HaCaT proliferation to 163% (p<0.01) at concentration of 50µg/mL. The L. glutinosa LMW fraction with highly stimulation effect is interesting for further studies. The increasing of cells proliferation of both plants extracts was along with their traditional uses for wound healing.