Planta Med 2012; 78 - PI148
DOI: 10.1055/s-0032-1320836

Anti-inflammatory compounds of Smilax corbularia Kunth

A Itharat 1, S Ruangnoo 1, S Makchuchit 1, P Thongdeeying 1, S Panthong 1
  • 1Applied Thai Traditional Medicine Centre, Faculty of Medicine, Thammasat University, Klongluang, Pathumthani, 12120 Thailand

Smilax corbularia Kunth (Smilacaceae) is Thai traditional medicine plant locally known as 'Hua-Khao-Yen Neua' were examined for their inhibitory activities against lipopolysaccharide (LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines. The ethanolic extract of Smilax corbularia Kunth exhibited the most inhibitory activity, with an IC50 value of 83.90µg/ml. From this extract, three compounds [engeletin (1) astilbin (2) and quercetin (3)] were isolated and further investigated for their inhibitory properties of NO production. It was found that 3 possessed the highest activity (IC50=9.17µg/ml), whereas 1 and 2 exhibited mild activity. The ethanolic extract, water extract of Smilax corbularia Kunth and its compounds 1–3 were also evaluated for the inhibitory effect on LPS-stimulated PGE2 release from RAW 264.7 cells. The results found that 1, 2 and 3 possessed potent activity against PGE2 release with IC50 values of 19.67, 19.72 and 19.85µg/ml respectively. The present study support the use of Smilax corbularia Kunth by Thai traditional medicine for treatment the inflammatory diseases