Planta Med 2012; 78 - PI136
DOI: 10.1055/s-0032-1320823

Inhibitory effect of ethanolic extract of Annona squamosa L. leaves on the expression of EGFR

C Ronpirin 1, T Charueksereesakul 2, V Thongrakard 2, T Tencomnao 3
  • 1Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, Thailand, 12121
  • 2Graduate Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  • 3Center of Excellence in Omics-Nano Medical Technology Development Project, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand

The precise causes of psoriasis, a relatively common, chronic, inflammatory and hyperproliferative skin disease, are not known, thus making it very difficult for therapy. We previously found an in vitro anti-psoriatic activity in ethanolic extract of Annona squamosa L. leaves. EGFR is overexpressed in psoriatic skin, thus considering as one of essential determinants of psoriasis. The objective of this study was to investigate the molecular effect of Annona squamosa L. leaf extract on the EGFR expression using HaCaT keratinocyte cell line as a model. Based on RT-PCR, concentrations at 3.15 and 1.575µg/mL (IC50=6.3µg/mL) significantly reduced the EGFR mRNA expression (P<0.05) although all three concentrations showed a similar tendency of inhibitory effect. Western blot analysis found that all concentrations of the extract tested greatly inhibited the expression of EGFR (P<0.05). Confocal immunofluorescence microscopy subsequently confirmed this finding. Taken together, this might suggest that the ethanolic extract of Annona squamosa leaves could exert its biological effect by suppressing the expression of EGFR biomarker.