Antioxidant and antihyperlipidemic activity of Tephrosia purpurea callus culture

This study was undertaken to investigation the effect of callus culture of Tephrosia purpurea (CCTeph) on blood glucose, tissue lipid profile, and lipidperoxidation in alloxan induced diabetes. The callus culture was successfully developed and maintained on MS medium supplemented with different concentrations and combinations of plant growth regulators. Aqueous extract of leaf and its callus culture were administered orally (300mg/kg body weight) for 21 days. The levels of lipid peroxides [TBARS, and Hydroperoxide] and tissue lipids [cholesterol, triglyceride, phospholipides and free fatty acids] blood glucose level was estimated in alloxan induced diabetic rats. The effects were compared with glibenclamide.Treatment with CCTeph and glibenclamide showed significant reduction in blood glucose level, in tissue lipids and lipid peroxide formation. The effect produced by CCTeph was comparable with that of glibenclamide. The decreased lipid peroxides and tissue lipids clearly indicate the antihyperlipidemic and antioxidant activity of CCTeph apart from its antidiabetic effect. The present study provided evidence first that CCTeph has Antihyperlipidemic and antioxidant activity, suggesting the potential of the tissue culture technique to substitute for wild Tephrosia purpurea in the pharmaceutical industry.