Planta Med 2012; 78 - PI126
DOI: 10.1055/s-0032-1320813

Chromatographic profiling and identification of two new iridoid-indole alkaloids by UPLC-MS and HPLC-SPE-NMR analysis of an antimalarial extract from Nauclea pobeguinii

YJ Xu 1, K Foubert 1, L Dhooghe 1, F Lemière 2, K Cimanga 1, 3, K Mesia 3, S Apers 1, L Pieters 1
  • 1Natural Products & Food – Research and Analysis, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, Antwerp, Belgium
  • 2Biomolecular Mass Spectrometry, Centre for Proteome Analysis and Mass Spectrometry (CeProMa), University of Antwerp, Groenenborgerlaan 171, Antwerp, Belgium
  • 3Faculty of Pharmaceutical Sciences, University of Kinshasa, P.O. Box 212, Kinshasa XI, Democratic Republic of Congo

The total 80% EtOH extract of stem bark of Nauclea pobeguinii (Rubiaceae), which is active against uncomplicated falciparum malaria as shown in previous clinical studies, was analysed by means of UPLC-MS and HPLC-SPE-NMR. Apart from the main constituent, strictosamide, a series of minor constituents was identified, including two new iridoid-indole alkaloids, i.e. naucleidinic acid (4) and 19-O-methyl-3,14-dihydroangustoline (7), together with 8 known iridoid-indole alkaloids, i.e. naucleidinal, magniflorine, naucleofficine D, two diastereoisomers of 3,14-dihydroangustoline, strictosidine, desoxycordifoline, 3α,5α-tetrahydrodeoxycordifoline lactam, and a phenol glycoside 3,4,5-trimethoxyphenol-β-D-apiofuranosyl-(1–6)-β-D-glucopyranoside (kelampayoside A).