Planta Med 2012; 78 - PI73
DOI: 10.1055/s-0032-1320760

Nucleoside antibiotic components from Streptomyces scopuliridis RB72

CW Choi 1, JS Choi 2, GH Yon 2, YK Ko 2, SY Ryu 2, CJ Kim 3, YI Kwon 4, YH Kim 1
  • 1College of Pharmacy, Chungnam National University, Daejeon 305–764, Korea
  • 2Korea Research Institute of Chemical Technology, Daejeon 305–600, Korea
  • 3Korea Research Institute of Bioscience and Biotechnology, Daejeon 305–333, Korea
  • 4Department of Food and Nutrition, Hannam University, Daejeon 305–811, Korea

A Streptomyces sp. strain producing antimicrobial compounds were isolated from woodland in Daejeon, Korea and identified as Streptomyces scopuliridis RB72 according to 16S rRNA analysis. This strain was cultured mass production in bennet's medium with Diaion HP-20. A new nucleoside antibiotic constituents herbicidin K (1) and three known nucleoside antibiotic constituents herbicidin A (2), herbicidin B (3), and herbicidin F (4) were isolated from ethyl acetate extracts of Streptomyces scopuliridis RB72. The structures of 1 - 4 were elucidated by extensive MS and NMR spectroscopic methods including 1H NMR, 13C NMR, 1H-1H COSY, HMQC, HMBC and NOESY. Antimicrobial activities of isolated compounds will be discussed.