Screening of Aspergillus nidulans metabolites from habitat mimicking media using LC-DAD-TOFMS system

A Klitgaard; DK Holm; JC Frisvad; KF Nielsen

doi:10.1055/s-0032-1320754

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Planta Med 2012; 78 - PI67
DOI: 10.1055/s-0032-1320754

Screening of Aspergillus nidulans metabolites from habitat mimicking media using LC-DAD-TOFMS system

A Klitgaard ¹, DK Holm ¹, JC Frisvad ¹, KF Nielsen ¹

¹Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark (DTU)

Congress Abstract

Fungi are a valuable source of metabolites and other bioactive compounds. These compounds are essential for human society, and it is estimated that around 49% of the drugs used to treat cancer are natural products or derived therefrom.

Six different wild types of Aspergillus nidulans have been cultured on a range of different habitat mimicking media. Extracts from the fungi were analyzed using a LC-DAD-TOFMS system, and by screening the extracts using an in-house database of known fungal metabolites. Using this database we were able to easily select unknown compounds, and dereplicate these using the spectrometric and spectroscopic data obtained from the analysis. The Analysis revealed several metabolites not previously reported in A. nidulans as well as a novel metabolite.