Identification of polyether antibiotics produced by the endophyte Streptomyces platensis RTd22

L Varella; EJ Crevelin; LAB de Moraes; ANG Dabul; ILBC Camargo; MT Pupo

doi:10.1055/s-0032-1320739

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Planta Med 2012; 78 - PI52
DOI: 10.1055/s-0032-1320739

Identification of polyether antibiotics produced by the endophyte Streptomyces platensis RTd22

L Varella ¹, EJ Crevelin ¹, LAB de Moraes ², ANG Dabul ³, ILBC Camargo ³, MT Pupo ¹

¹School of Pharmaceutical Sciences of Ribeirão Preto – University of São Paulo (USP). Ribeirão Preto, SP, Brazil
²School of Philosophy, Sciences and Letters of Ribeirão Preto, USP. Ribeirão Preto, SP, Brazil
³Physics Institute of São Carlos, USP. São Carlos, SP, Brazil

Congress Abstract

The endophytic actimomycete S. platensis RTd22 was cultured in rice solid medium and shaken ISP-2 liquid medium for different periods of time. Extracts and sub-fractions obtained after the chromatographic procedures showed remarkable activity against Staphylococcus aureus ATCC 6538, S. saprophyticus ATCC 15305 and S. aureus ATCC 29213. Some samples from both culture media also showed high activity against the antibiotic-resistant bacteria: vancomycin-intermediate S. aureus Mu50 (VISA), methicillin-resistant S. aureus SA16 (MRSA) and vancomycin-resistant Enterococcus faecium VRE16. Analyses by ESI-MS (AcquityTM UPLC/Waters) of the active fractions allowed the rapid identification of the ionophore antibiotics grisorixin (1), nigericin (2), and mutalomycin (3), structurally related polyether polyketides. The structural identification of 1–3 together with the antibiotic activities will be presented.