HT-MALDI-MS: A keystone for Brazilian biodiversity conservation

In this work we provide a HT-MALDI-MS forest screening method to provide added value to local specimens of plants. Ionic liquid was used to get around known ionization problems. Our strategy is based on the ability of MALDI to quickly screen a very large number of samples using an ionic liquid as matrix. Using this approach, we can screen up to 400 samples within a 6 hour time-frame. Finding plants with the correct signatures, indicating candidate molecules that have commercial value, are then prioritized for the lower throughput but quantitative LC-MS studies where the retention time, UV and tandem MS signatures are used to confirm the identity of the molecule. Thus, the screening of an entire forest can be accomplished in a short time, which is currently not feasible with other methodologies. Our current forest dereplication revealed high levels of alkaloids on leaves sampled at SMSP, with emphasis to strictosidine (>5%) in Simira sampaioana (Rubiaceae). This is the starter of indolic alkaloid synthesis, such as Vincristine. The occurrence of such high levels of its key biosynthesizer represents a big opportunity for forest inhabitants to profit on yielding leave's compounds by single solvent-recrystallization methods, and making money furnishing this compound for a spin of company.