Planta Med 2012; 78 - PD179
DOI: 10.1055/s-0032-1320537

Sesamin from the roots of YErba mansa (Anemposis californica)

RO Bussey III 1, NB Cech 1, AA Sy-Cordero 1, JO Falkinham 2, NH Oberlies 1
  • 1Department of Chemistry and Biochemistry, The University of North Carolina at Greensboro, Greensboro, NC 27402
  • 2Department of Biological Sciences, Virginia Polytechnic Institute & State University, Blacksburg, VA 24061

Anemopsis californica, a plant native to the southwestern United States and Northern Mexico, has a long history of use by Native Americans to treat infection. The goal of these studies was to identify chemical constituents of this sparsely studied plant. A crude extract of A. californica was shown to inhibit the growth of clinical isolates of Mycobacterium avium and Mycobacterium marinum. This extract underwent several steps of bioactivity-directed fractionation and purification with normal and reversed phase chromatography. Sesamin (1), a tetrahydrofuran derivative, was isolated from the active fractions, and characterized by NMR spectroscopy and X-ray crystallography. The compound demonstrated a MIC of 20µg/mL against several mycobacterium species. This is the first report of sesamin produced by a plant in the Saururaceae plant family. Efforts to characterize additional bioactive compounds from A. californica are ongoing.