Antibacterial activity of the methanolic extract of Hyptis atrorubens (Lamiaceae)
The leaves and stems of Hyptis atrorubens Poit. (Lamiaceae) were collected in Guadeloupe. The methanol extract was tested at six concentrations (10mg/mL, 5mg/mL, 2.5mg/mL, 1.2mg/mL, 0.6mg/mL and 0.3mg/mL) against a panel of pathogenic Gram-positive and Gram-negative bacteria in vitro. This methanolic extract demonstrated antibacterial activity against two Gram-positive bacteria (Staphylococcus epidermidis and Enterococcus faecalis), and two Gram-negative bacteria (Burkholderia cepacia and Stenotrophomonas maltophilia) at all of the concentrations. Bioautography enabled the obtention and identification of two antibacterial compounds from this plant: rosmarinic acid and methyl rosmarinate. This work supports the recognition of H. atrorubens, a traditional remedy in French West Indies, as a medicinal plant in France.