Antioxidant, anticancer and apoptotic inducer activities of piperaceae extracts on hela cells line

Background: Our research is to explore the herbal medicine as an alternative therapy for obtaining chemotherapeutic drugs base on the antioxidant, cytotoxic and apoptotic inducer activities. Piperaceae including Piper betle L., Piper cf. fragile Benth, Piper umbellatum L., Piper aduncum L., Piper pellucidum L. are popularly used as herbal efficacy. The objective: this research was conducted to elucidate the cytotoxic and apoptotic inducer activities on HeLa cell line and antioxidant of Piperaceae water extracts. Method: the cytotoxic activity was determined by inhibiting proliferations cells and apoptotic activity was determined with Sub-G1 flowcytometric, the antioxidant activity was determined by using Superoxide Dismutase (SOD) value and Inhibitory Concentration-median (IC-50) of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Results: The IC-50 of cytotoxic for 24 hours incubation were 7.13; 2.93; 6.71; 3.91; 2.85µg/ml respectively and Cisplatin 0.07µg/ml; 48 hours incubation were lower than 24 hours incubation. The highest apoptotic inducer for 24 hours and 48 hours were P. betle 12.5µg/ml, P. cf. fragile 50µg/ml, P. umbelatum 25µg/ml, P. aduncum 100µg/ml, Cisplatin 8µg/m. The highest SOD activity was P. betle extract 108.01 U/ml. The IC-50 of DPPH scavenging activity respectively were 5.56; 52.49; 15.36; 102.84; 9.00µg/ml. Conclusions: P. betle extract has the highest antioxidant activities, all Piperaceae extracts has high cytotoxic. P betle extract exhibit the highest apoptotic inducer activity.