Planta Med 2012; 78 - PD115
DOI: 10.1055/s-0032-1320473

Anti-inflammatory activity from new molecules derived from Convolutamydine A

YL Lisbôa 1, CC Guilhon 1, MR Gonçalves 1, B Silva 2, AC Pinto 2, PD Fernandes 1
  • 1Federal University of Rio de Janeiro, Institute of Biomedical Science
  • 2Institute of Chemistry. Brazil

Convolutamydine A (4,6-dibromo-3hidroxyoxindole) is a substance isolated from the marine bryozoans Amathia convoluta and have several biological effects. The aim of this work was to evaluate the anti-inflammatory properties of three molecules synthesized from convolutamydine A in the carrageenan (Carr)-induced inflammation into the subcutaneous air pouch (SAP). Male mice (20–25g) received oral administration of I-158, I-160 or I-161 (1 or 10mg/kg) 1h before Carr injection. After 24h the mice were euthanized and the exsudate from SAP were collected to several measurements (listed below). Authorization for animals' assays was ICBDFBC015. Statistical analyses was performed by ANOVA and Bonferroni's test (*p<0.05). Results are:

I-158

I-160

I-161

PBS

Carr

1

10

1

10

1

10

Cell

2.9±1

63.1±12

53±9

44±9

29±8*

57±9

33±3*

48±8

NO (µM)

20±7

173±14

79±7*

112±42*

86±14*

28±6*

148±10

41±13*

TNF-α (pg/mL)

61.1±3

637±53

340±21*

281±42*

391±57*

190±36*

240±81*

382±57*

Conclusions: All three substances demonstrated significant anti-inflammatory effect being I-160 the most promising to future assays.

Financial support: CNPq, FAPERJ, Instituto Vital Brazil.