Planta Med 2012; 78 - PD112
DOI: 10.1055/s-0032-1320470

Evaluation of Tagetes patula L. (Asteraceae) against filamentous and entomopathogenic fungi

FAS Politi 1, VYM Watanabe 1, R Sorrechia 1, GM Figueira 2, RCLR Pietro 1
  • 1School of Pharmaceutical Sciences, UNESP- Univ Estadual Paulista, Araraquara, São Paulo, Brazil
  • 2Chemical, Biological and Agricultural Pluridisciplinary Research Center, University of Campinas, Campinas, SP, Brazil

Motivated by the search for a natural product for controlling pathogenic fungal species related to the habits of dogs and cats, without interfering in the growth of entomopathogenic fungi, responsible for the biological control of ticks, were tested extracts of Tagetes patula L. (Asteraceae) against strains of Trichophyton rubrum, Microsporum canis, Trichophyton mentagrophytes and entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana. The minimum inhibitory concentrations (MIC) was determined by microdilution plate method for 70% ethanol extracts obtained by percolation of the aerial parts (PAEtOH70), aerial parts without flowers (PASFEtOH70) and flowers (FlEtOH70). Amphotericin B was a positive control. The results revealed that all extracts were ineffective against the entomopathogenic fungi, showing MIC values at the highest dilution tested (1250 ug/mL), except PASFEtOH70 (650 ug/mL). Against dermatophytes were obtained significant activities of FlEtOH70 against M. canis and T. rubrum (193.3 ug/mL and 253.9 ug/mL, respectively) and an interesting activity of PAEtOH70 against T. rubrum (312.5 ug/mL). These results suggest a selective action against pathogenic fungi, allowing future studies of the synergistic action of extracts of T. patula with entomopathogenic fungi. Support: FAPESP