Planta Med 2012; 78 - PD80
DOI: 10.1055/s-0032-1320438

Vasorelaxant effects of 1-nitro-2-phenylethene in isolated rings of rat aorta

LA Barbosa 1, FCVS Neto 2, KMS Rodrigues 2, JH Leal-Cardoso 2, RS Borges 3, PJC Magalhães 1, S Lahlou 1
  • 1Federal University of Ceará, 60430–275
  • 2State University of Ceará, 60740–000, Fortaleza, CE, Brazil
  • 3Federal University of Pará, 66075–900, Belém, PA, Brazil

Nitro-2-phenylethane (NPEa) is a vasorelaxant constituent of the essential oil of Aniba canelilla. Here, we investigated whether 1-nitro-2-phenylethene (NPEe), a derivative molecule obtained synthetically, has similar properties in isolated rings of rat aorta. Trace recordings were obtained isometrically. NPEe (0.1–100µg/ml) had no effect on aortic rings under resting tonus, but relaxed sustained contractions induced by phenylephrine (PHE, 1µM) in endothelium-intact preparations (IC50=6.01±1.62µg/ml; n=6). This vasorelaxation remained unaltered by vascular endothelium removal (IC50=4.56±0.75µg/ml; n=5), but was significantly reduced by pretreatment with 10µM ODQ (IC50=26.18±7.54µg/ml, n=9). NPEe also relaxed the contractions evoked by 60 mM K+ with the same potency for relaxing PHE without influence of endothelium removal. In Ca2+-free medium, NPEe (1–3µg/ml) inhibited contractions induced by exogenous Ca2+ (0.1–20 mM) addition in preparations depolarized by K+, but was inert against PHE-induced phasic contractions in presence of verapamil (1µM; n=9). NPEe inhibited contractions caused by extracellular Ca2+ restoration in thapsigargin-treated aorta in Ca2+-free medium. Thus, as NPEa, NPEe has vasodilator properties in rat aortic rings, which seem potentially mediated through a guanylate cyclase pathway.