Planta Med 2012; 78 - PD52
DOI: 10.1055/s-0032-1320410

Dehydroeburicoic acid, an antileukemic triterpene from the fruiting bodies of dish-cultured Antrodia Cinnamomea

KH Lai 1, YC Du 1, MC Lu 1, 2, TY Wu 1, YM Hsu 1, YC Lin 1, M El-Shazly 1, TS Chu 3, CF Chen 3, FR Chang 1, YC Wu 1, 4
  • 1Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan
  • 2Graduate Institute of Marine Biotechnology, National Dong Hwa University, Pingtung, Taiwan
  • 3Yung Peng Biotechnology. CO., LTD, Taiwan
  • 4School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan

Antrodia cinnamomea (AC), an endemic species, has long been used as a chemopreventive mushroom in traditional Taiwanese medicine. In this current study, we reported a standardized triterpenoids-dependent analysing platform for marketing AC products. Twelve different AC products were collected and evaluated for their triterpenoids content. The results revealed that fruiting bodies from dish-cultures (AC-DC2) had a similar triterpenoids profile compared to wild AC. The AC-DC2 ethanolic extract was subjected to bioactivity-guided isolation and yielded five major cytotoxic triterpenes (1-5). Among them, dehydroeburicoic acid (5) was the most potent cytotoxic component against HL-60 cells while it activated DNA damage, apoptosis biomarkers and also inhibited topoisomerase II. In the xenograft animal model, 5 resulted in a marked decrease of tumour weight and size without any significant decrease in mice body weights. Taken together, our results showed that fruiting bodies from the dish-cultured AC, which had the same major active triterpenoids compared to wild AC, could be considered as one of alternative AC sources.