Effect of hydro-alcoholic extract from Brunella and Adiante Capillaire on calcium oxalate crystallization in-vitro

Use of herbal medicine is a remedial method for treatment of kidney stones in Iranian traditional medicine. In order to being a therapeutic-hygienic problem for treatment of kidney stone, using herbal medicine will be effective. Brunella and Adiante capillaire are two medicinal plants which had been used for kidney disorders in traditional references. In this study effect of hydro-alcoholic extract of these two herbs on crystallization of calcium oxalate in vitro were investigated. Extracts of every herb were provided in the concentrations of 0.25, 1 and 2mg/ml, at first. Urine samples of 10 adult and healthy persons devided in two groups (by and without adding of extracts). Presence of calcium oxalate crystals were evaluated immediately and 24 hours after induction. Extracts of Brunella has been decreased number of crystals against control group in 0.25, 0.5 and 1mg/ml concentrations (P<0.05) and increased number of mono hydrate calcium optical but Adiante capillaire has not (P>0.05). Index of turbidity by spectrophotometry in none of extracts showed significant difference (P>0.05). Extract of Brunella lead to prevention of composition of crystals and it can inhibit creation of kidney stone probably by increase in number of mono hydrate crystals. Animal studies for confirmation of this effect will be offered.