Protective activity of Styrax camporum extract against genotoxicity induced by doxorubicin and methyl methanesulfonate
Styrax camporum Pohl is a tall shrub or a tree with small white flowers, which grows in the states of São Paulo and Minas Gerais and is popularly used for the treatment of gastroduodenal diseases. The aim of this study was to evaluate the genotoxic potential of S. camporum hydroalcoholic extract and its influence on genotoxicity induced by doxorubicin (DXR) and methyl methanesulfonate (MMS) in Swiss mice using the micronucleus and comet assays, respectively. The animals were treated with different doses of the extract (250, 500 and 1000mg/kg body weight). For antigenotoxicity assessment, different doses of the S. camporum extract were administered simultaneously with DXR and MMS. The results showed that the S. camporum extract itself was not genotoxic. The number of micronucleus was significantly lower in animals treated with the S. camporum extract and DXR when compared to animals treated only with DXR. In the comet assay, the S. camporum extract significantly reduced the extent of DNA damage in liver cells induced by MMS. The antioxidant activity of the active compounds of S. camporum extract may explain the effect.
Financial support: FAPESP; Grant number 2011/21310–2