Planta Med 2012; 78 - PB14
DOI: 10.1055/s-0032-1320343

Elucidation of the biosynthesis of meroterpenoid yanuthone D in Aspergillus Niger

DK Holm 1, LM Petersen 1, A Klitgaard 1, Z Jarczynska 1, TO Larsen 1, UH Mortensen 1
  • 1Center for Microbial Biotechnology, Department of Systems Biology, Technical University of Denmark, Soltofts Plads – Building 223, DK-2800 Kgs. Lyngby

We have elucidated the mode of biosynthesis of the meroterpenoid compound Yanuthone D in Aspergillus niger. We have successfully deleted all cluster genes, and identified a number of intermediates. Structures of the intermediates were solved using a combined approach comprising classical 1D- and 2D-NMR and tandem mass spectrometry (MS/MS). In this study we have confirmed that Yanuthone D is of meroterpenoid origin, and we have identified an unexpected precursor, which has not before been reported for Aspergillus niger.