Pulegone inhibits selectively butyrylcholinesterase and ameliorates memory in rats

RM Marçal; C Benetti; AT Costa; JKA Lima; LB Couto; PB Alves; AF Blank; SC França; RPM Fernandes

doi:10.1055/s-0032-1320275

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Planta Med 2012; 78 - CL40
DOI: 10.1055/s-0032-1320275

Pulegone inhibits selectively butyrylcholinesterase and ameliorates memory in rats

RM Marçal ¹, C Benetti ¹, AT Costa ¹, JKA Lima ¹, LB Couto ², PB Alves ¹, AF Blank ¹, SC França ², RPM Fernandes ¹

¹LAPHET, DFS, Universidade Federal de Sergipe; Av: Marechal Rondon, S/N, CEP 49100–000, São Cristóvão, SE, Brazil
²Laboratory of Biotechnology, UNAERP, Av. Costábile Romano, 2.201, CEP 14096–900, Ribeirão Preto, SP, Brasil

Congress Abstract

In a search for new natural compounds for treating AD cognitive symptoms, we investigated the anticholinesterase activity of pulegone, a monoterpene ketone found in several essential oils. Cholinesterase inhibition was evaluated by the Elmman's method. Pulegona potency (IC₅₀), maximal inhibition (E_max), and selectivity (IC_50AChE/IC_50BuChE) have been evaluated for AChE and BuChE inhibition in vitro (A). The mode of BuChE inhibition by pulegone has been investigated (B). The effect of pulegone in the scopolamine induced learning deficit in the passive avoidance test in rat (C) and the level of brain cholinesterase inhibition (D) after oral administration was also measured. The results of in vitro, in vivo and ex vivo experiments will be presented.