Planta Med 2012; 78(15): 1636-1638
DOI: 10.1055/s-0032-1315208
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

Inhibition of Herpes Simplex Virus Type 1 by Thymol-Related Monoterpenoids

Wen-Lin Lai
1  School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, Taiwan
2  Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan
,
He-Shing Chuang
3  Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan
,
Meng-Hwan Lee
4  Division of Biotechnology, Animal Technology Institute Taiwan, Miaoli, Taiwan
5  Yangsen Biotechnology Co., Ltd., Taipei, Taiwan
,
Chia-Li Wei
5  Yangsen Biotechnology Co., Ltd., Taipei, Taiwan
6  Department of Biochemical Science & Technology, National Chiayi University, Chiayi, Taiwan
,
Chin-Fu Lin
7  Department of Clinical Microbiology Laboratory, Taichung Veterans General Hospital, Taichung, Taiwan
,
Ying-Chieh Tsai
3  Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan
› Author Affiliations
Further Information

Publication History

received 10 February 2012
revised 17 July 2012

accepted 18 July 2012

Publication Date:
13 August 2012 (online)

Abstract

This study examined the anti-herpes simplex virus type I activity of the major constituents of several essential oils. Plaque reduction assays were performed to evaluate anti-herpes simplex virus type I activity. Thymol and carvacrol both possessed significant antiviral activity with an IC50 of 7 µM, and herpes simplex virus type I was 90 % inactivated within 1 hr. The mode of antiviral action was shown to affect the virion directly. Evidence was also observed by electron microscopy. Evaluation of the structural requirements for antiviral activity of thymol-related monoterpenoids revealed that aliphatic side chains had a minor effect, while a hydrophilic group on the benzene ring was sufficient for activity. Our results suggest that thymol and carvacrol are potential candidates for topical therapeutic application to reduce herpes simplex virus transmission.

Supporting Information