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Semin Thromb Hemost 1995; 21(S 02): 96-102
DOI: 10.1055/s-0032-1313610
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Influence of HPA-1a and HLA Antibodies on Primary Hemostasis

Volker Kretschmer
,
Bernd Huss
,
Kristine Feller
,
Norbert Ponath
,
Volker Kiefel
Further Information

Publication History

Publication Date:
09 May 2012 (online)

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Abstract

The influence of platelet specific and HLA antibodies was investigated on primary hemostasis by the in vitro bleeding test (IVBT) (Thrombostat 4000).

Seven of 12 plasmas with HPA-la (PIA1) antibodies and two with antibodies against glycoprotein Ib/IX significantly inhibited the IVBT of normal, cross-match-positive donor blood. This correlated with a significant increase ofGMP-I40 (CD 62) on the platelets, determined by flow cytometry. These results agree with the literature showing both activation and inhibition of platelet function by platelet specific antibodies. On the other hand, HLA antibodies showed a similar platelet activation (shortening of occlusion time and decrease in blood volume in the IVBT, increase of CD 62 expression) as plasma samples of polytransfused thrombocytopenic patients without HLA antibodies. It can be concluded that neither an activating nor an inhibitory effect of HLA antibodies on platelet function is significant in vivo. The IVBT seems not to be suited for compatibility testing.

Keywords

platelet specific antibodies - anti-PIA1 - HLA antibodies - platelet function - Thrombostat
 

  • References

  • 1 De Gaetano G, Vermylen J, Verstrate M. Platelet aggregation by a specific human immunoglobulin. Thromb Diath Haemorrh 24: 419-431 1970;
    PubMedGoogle Scholar
  • 2 Mueller-Eckhardt C, Mönch H. The effect of platelet-specific alloantibodies on the aggregation of human thrombocytes induced by ADP and collagen. Thromb Diath Haemorrh 25: 379-380 1971;
    PubMedGoogle Scholar
  • 3 Wu KK, Thompson JS, Koepke JA, Hoak JC, Flink R. Heterogeneity of antibody response to human platelet transfusions. J Clin Invest 58: 432-438 1976;
    CrossrefPubMedGoogle Scholar
  • 4 Heinrich D, Stephinger U, Mueller-Eckhardt C. Specific interaction of HLA antibodies (eluates) with washed platelets. Br J Haematol 35: 441-452 1977;
    CrossrefPubMedGoogle Scholar
  • 5 Clnes DB, Schreiber AD. Effect of anti-PLA1 antibody on human platelets. The role of complement. Blood 53: 567-577 1979;
    PubMedGoogle Scholar
  • 6 Schreiber AD, Cines DB, Zmijewski C, Colman RW. Effect of anti-PlA1 antibody on human platelets. II. Mechanism of the complement dependent release reaction. Blood 53: 578-587 1979;
    PubMedGoogle Scholar
  • 7 Leeuwen van EF, Leeksma OC, van Mourik JA, Engelfriet CP, Kr von dem Borne AEG. Effect of binding of anti-Zwa antibodies on platelet function. Vox Sang 47: 280-289 1984;
    CrossrefPubMedGoogle Scholar
  • 8 Ryu T, Davis JM, Schwartz KA. Dose-dependent platelet stimulation and inhibition induced by anti-PlAl IgG. J Lab Clin Med 116: 91-99 1990;
    PubMedGoogle Scholar
  • 9 Christie DJ, Swinehart CD. Human platelet activating antibodies. Semin Thromb Hemost 18: 186-192 1992;
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Kratzer MAA, Born GVR. Simulation of primary haemostasis in vitro. Haemostasis 15: 357-362 1985;
    PubMedGoogle Scholar
  • 11 Kratzer MAA, Bellucci S, Caen JP. Detection of abnormal platelet function with an in vitro model of primary haemostasis. Haemostasis 15: 363-370 1985;
    PubMedGoogle Scholar
  • 12 Kretschmer V, Schikor B, Söhngen D, Dietrich G. In vitro bleeding test - a simple method for the detection of aspirin effects on platelet function. Thromb Res 56: 593-602 1989;
    CrossrefPubMedGoogle Scholar
  • 13 Dietrich G, Weber D, Kretschmer V. The in vitro bleeding test: standardization of the methodical procedure. Lab Med 17: 317-323 1993;
    PubMedGoogle Scholar
  • 14 Dietrich G, Kretschmer V, Orth D, Haupt W. Primary hemostasis in hemodilution - 1. Hematocrit. Infusionstherapie 17: 212-213 1990;
    PubMedGoogle Scholar
  • 15 Dietrich G, Orth D, Haupt W, Kretschmer V. Primary hemostasis in hemodilution - 2. Infusion solutions. Infusionstherapie 17: 214-216 1990;
    PubMedGoogle Scholar
  • 16 Kretschmer V, Huss B, Dietrich G, Heymanns J, Pflüger KH. Determination of bleeding risk in thrombocytopenic patients receiving platelet substitution. Transfus Sci 14: 27-34 1993;
    CrossrefPubMedGoogle Scholar
  • 17 Teraski PI, McClelland JD. Microdroplet assay of human serum cytotoxins. Nature 204: 998-1000 1962;
    PubMedGoogle Scholar
  • 18 Schneider W, Schnaidt M. The platelet adhesion immunofluorescence test: a modification of the platelet suspension immunofluorescence test. Blut 43: 389-392 1981;
    CrossrefPubMedGoogle Scholar
  • 19 Kiefel V, Santoso S, Weisheit M, Mueller-Eckhardt C. Monoclonal antibody-specific immobilization of platelet antigens (MAIPA): A new tool for the identification of platelet reactive antibodies. Blood 70: 1722-1726 1987;
    PubMedGoogle Scholar
  • 20 Colman RW, Schreiber AD. Effect of heterologous antibody on human platelets. Blood 48: 119-131 1976;
    PubMedGoogle Scholar
  • 21 Falus a, Merétey K, Bagdi D, Diószegi M, Böhm U, Csák E, Bozsóky S. Beta 2-microglobulin-specific autoantibodies cause platelet aggregation and interfere with ADP-induced aggregation. Clin Exp Immunol 47: 103-109 1982;
    PubMedGoogle Scholar
  • 22 Duncan JR, Rosse WF. Alloantibody-induced platelet serotonin release is blocked by antibody to the platelet PLAlantigen. Br J Haematol 64: 331-338 1986;
    CrossrefPubMedGoogle Scholar
  • 23 Cosgrove IJ, Vaughan HA, Tjandra IJ, Thurbow PJ, McKenzie IFC. HLA (class I) antigens on platelets are involved in platelets function. Immunol Cell Bioi 66: 69-77 1988;
    PubMedGoogle Scholar
  • 24 Christie DJ, Leja DN, Carlson DL, Swinehart CD. Characterization of platelet activation induced by HLA antibodies associated with alloimmune thombocytopenia. J Lab Clin Med 121: 437-443 1993;
    PubMedGoogle Scholar
  • 25 Mueller-Eckhardt C. Platelet allo- and autoantigens and their clinical implications. In: Nance SJ, (ed) Transfusion Medicine in the 1990's . Arlington, VA: American Association of Blood Banks; 1990: 63-93
    Google Scholar
  • 26 Hirschman RJ, Schulman NR. The use of platelet serotnin release as a sensitive method for detecting antiplatelet antibodies and a plasma antiplatelet factor in patients with idopathic thrombocytopenic purpura. Br J Haematol 24: 793-802 1973;
    CrossrefPubMedGoogle Scholar
  • 27 Deykin D, Hellerstein LJ. The assessment of drug-dependent and isoimmune antiplatelet antibodies by the use of platelet aggregometry. J Clin Invest 51: 3142-3153 1972;
    CrossrefPubMedGoogle Scholar
  • 28 Lee H, Nurden AT, Thomaidis A, Caen JP. Relationship between fibrinogen binding and the platelet glycoprotein deficiencies in Glanzmann's thrombasthenia type I and type II. Br J Haematol 48: 47-57 1981;
    PubMedGoogle Scholar
  • 29 Levy-Toledano S, Tobelem G, Legrand C, Bredoux R, Degos L, Nurden A, Caen JP. Acquired IgG antibody occuring in a thrombasthenic patient: its effect on human platelet function. Blood 51: 1065-1071 1978;
    PubMedGoogle Scholar
  • 30 Newman PJ, McEver RP, Doers MP, Kunicki TJ. Synergistic action of two murine monoclonal antibodies that inhibit ADPinduced platelet aggregation without blocking fibrinogen binding. Blood 69: 668-676 1987;
    PubMedGoogle Scholar
  • 31 Modderman PW, Huisman HG, van Mourick JA, von dem Borne AEGK. A monoclonal antibody to the human platelet glycoprotein IIb/IIIa complex induces platelet activation. Thromb Haemost 60: 68-74 1988;
    PubMedGoogle Scholar
  • 32 Clancy R, Jenkins E, Firkin B. Qualitative platelet abnormalities in idiopathic thrombocytopenic purpura. N Engl J Med 286: 622-626 1972;
    CrossrefPubMedGoogle Scholar
  • 33 Zahavi J, Marder VJ. Acquired “storage pool disease” of platelets associated with circulating antiplatelet antibodies. Am J Med 56: 883-890 1974;
    CrossrefPubMedGoogle Scholar
  • 34 Regan MG, Lackner H, Karpatkin S. Platelet function and coagulation profile in lupus erythematosus. Ann Intern Med 81: 462-468 1974;
    PubMedGoogle Scholar
  • 35 Lackner H, Karpatkin S. On the “easy bruising” syndrome with normal platelet count. Ann Intern Med 83: 190-195 1975;
    PubMedGoogle Scholar
  • 36 Heyns A, du P, Fraser J, Retief FP. Platelet aggregation in chronic idiopathic thrombocytopenic purpura. J Clin Pathol 31: 1239-1243 1978;
    CrossrefPubMedGoogle Scholar