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Int J Sports Med 2013; 34(01): 1-7
DOI: 10.1055/s-0032-1312584
Physiology & Biochemistry
© Georg Thieme Verlag KG Stuttgart · New York

Short-Term Supplementation with Alpha-Ketoglutaric Acid and 5-Hydroxymethylfurfural Does not Prevent the Hypoxia Induced Decrease of Exercise Performance Despite Attenuation of Oxidative Stress

H. Gatterer
1   Department of Sport Science, University of Innsbruck, Innsbruck, Austria
,
J. Greilberger
2   Department of Physiological Chemistry, Medical University Graz, Graz, Austria
3   Schwarzl Medical Center, Institut für Laborwissenschaften Dr. Greilberger, Graz, Austria
,
M. Philippe
1   Department of Sport Science, University of Innsbruck, Innsbruck, Austria
,
M. Faulhaber
1   Department of Sport Science, University of Innsbruck, Innsbruck, Austria
,
R. Djukic
3   Schwarzl Medical Center, Institut für Laborwissenschaften Dr. Greilberger, Graz, Austria
,
M. Burtscher
1   Department of Sport Science, University of Innsbruck, Innsbruck, Austria
› Author Affiliations
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Publication History



accepted after revision 13 March 2012

Publication Date:
14 August 2012 (online)

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Abstract

Reactive oxygen species are thought to partly be responsible for the hypoxia induced performance decrease. The present study evaluated the effects of a broad based antioxidant supplementation or the combined intake of alpha-ketoglutaric acid (α-KG) and 5-hydroxymethylfurfural (5-HMF) on the performance decrease at altitude. 18 healthy, well-trained males (age: 25±3 years; height: 179±6 cm; weight: 76.4±6.8 kg) were randomly assigned in a double-blind fashion to a placebo group (PL), a α-KG and 5-HMF supplementation group (AO1) or a broad based antioxidant supplementation group (AO2). Participants performed 2 incremental exercise tests to exhaustion on a cycle ergometer; the first test under normoxia and the second under hypoxia conditions (simulated altitude, FiO2=13% ~ 4 300 m). Supplementation started 48 h before the hypoxia test. Maximal oxygen uptake, maximal power output, power output at the ventilatory and lactate threshold and the tissue oxygenation index (NIRS) were measured under both conditions. Oxidative stress markers were measured before the supplementation and after the hypoxia test. Under hypoxia conditions all performance parameters decreased in the range of 19–39% with no differences between groups. A significant change from normoxia to hypoxia (p<0.001) and between groups (p=0.038) were found for the tissue oxygenation index. Post hoc test revealed significant differences between the PL and both, the AO1 and the AO2 group. The oxidative stress parameter carbonyl protein changed from normoxia to hypoxia in all participants and 4-hydroxynonenal decreased in the AO1 group only. In conclusion the results suggest that short-term supplementation with an antioxidant does not prevent the performance decrease at altitude. However, positive effects on muscle oxygen extraction, as indicated by the tissue oxygenation index, might indicate that mitochondrial functioning was actually influenced by the supplementation.

Key words

altitude - antioxidants - submaximal performance - reactive oxygen species - ventilatory threshold - maximal oxygen uptake
 

  • References

  • 1 Amann M, Subudhi AW, Foster C. Predictive validity of ventilatory and lactate threshold for cycling time trial performance. Scand J Med Sci Sports 2006; 16: 27-34
    CrossrefPubMedGoogle Scholar
  • 2 Askew EW. Work at high altitude and oxidative stress: antioxidant nutrients. Toxicology 2002; 180: 107-119
    CrossrefPubMedGoogle Scholar
  • 3 Boushel R, Langberg H, Olesen J, Gonzales-Alonzo J, Bülow J, Kjaer M. Monitoring tissue oxygen availability with near infrared spectroscopy (NIRS) in health and disease. Scand J Med Sci Sports 2001; 11: 213-222
    CrossrefPubMedGoogle Scholar
  • 4 Burtscher M, Faulhaber M, Flatz M, Likar R, Nachbauer W. Effects of short-term acclimatization to altitude (3 200 m) on aerobic and anaerobic exercise performance. Int J Sports Med 2006; 27: 629-635
    Article in Thieme ConnectPubMedGoogle Scholar
  • 5 Buskirk ER, Kollias J, Picon-Reategui E, Akers R, Prokop E, Baker P. Physiology and performance of track athletes at various altitudes in the United States and Peru. In: Goddard RF. ed. The Effects of Altitude on Physical Performance. Athletic Institute; Chicago, IL: 1966: 65-72
    Google Scholar
  • 6 Calbet JAL, Lundby C. Air to muscle O2 delivery during exercise at altitude. High Alt Med Biol 2009; 10: 123-134
    CrossrefPubMedGoogle Scholar
  • 7 Cerretelli P. Limiting factors to oxygen transport on Mount Everest. J Appl Physiol 1976; 40: 658-667
    PubMedGoogle Scholar
  • 8 Esterbauer H, Schaur RJ, Zollner H. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radic Biol Med 1991; 11: 81-128
    CrossrefPubMedGoogle Scholar
  • 9 Ferretti G, Moia C, Thomet JM, Kayser B. The decrease of maximal oxygen consumption during hypoxia in man: a mirror image of the oxygen equilibrium curve. J Physiol 1997; 498: 231-237
    PubMedGoogle Scholar
  • 10 Fulco CS, Rock PB, Cymerman A. Maximal and submaximal exercise performance at altitude. Aviat Space Environ Med 1998; 69: 793-801
    PubMedGoogle Scholar
  • 11 Gross M, Baum O, Hoppeler H. Antioxidant supplementation and endurance training: Win or loss?. Eur J Sport Science 2011; 11: 27-32
    PubMedGoogle Scholar
  • 12 Harriss DJ, Atkinson G. Update – ethical standards in sport and exercise science research. Int J Sports Med 2011; 32: 819-821
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Howley ET, Bassett DR, Welch HG. Criteria for maximal oxygen uptake: review and commentary. Med Sci Sports Exerc 1995; 27: 1292-1301
    PubMedGoogle Scholar
  • 14 Ischiropoulos H. Biological tyrosine nitration: a pathophysiological function of nitric oxide and reactive oxygen species. Arch Biochem Biophys 1998; 1: 1-11
    PubMedGoogle Scholar
  • 15 Jammes Y, Steinberg JG, Bregeon F, Delliaux S. The oxidative stress in response to routine incremental cycling exercise in healthy sedentary subjects. Respir Physiol Neurobiol 2004; 144: 81-90
    CrossrefPubMedGoogle Scholar
  • 16 Lamprecht M, Greilberger J, Oettl K. Analytical aspects of oxidatively modified substances in sports and exercises. Nutrition 2004; 20: 728-730
    CrossrefPubMedGoogle Scholar
  • 17 Levine BD. VO2max: what do we know, and what do we still need to know?. J Physiol 2008; 586.1: 25-34
    PubMedGoogle Scholar
  • 18 Li MM, Wu LY, Zhao T, Xiong L, Huang X, Liu ZH, Fan XL, Xiao CR, Gao Y, Ma YB, Chen JJ, Zhu LL, Fan M. The protective role of 5-HMF against hypoxic injury. Cell Stress Chaperones 2011; 16: 267-273
    CrossrefPubMedGoogle Scholar
  • 19 Li YX, Li Y, Qian ZJ, Kim MM, Kim SK. In vitro antioxidant activity of 5-HMF isolated from marine red alga Laurencia undulata in free-radical-mediated oxidative systems. J Microbiol Biotechnol 2009; 19: 1319-1327
    PubMedGoogle Scholar
  • 20 Magalhaes J, Ferreira R, Neuparth MJ, Oliveira PJ, Marques F, Ascensao A. Vitamin E prevents hypobaric hypoxia-induced mitochondrial dysfunction in skeletal muscle. Clin Sci 2007; 113: 459-466
    CrossrefPubMedGoogle Scholar
  • 21 Mancini DM, Bolinger L, Li H, Kendrick K, Chance B, Wilson JR. Validation of near-infrared spectroscopy in humans. J Appl Physiol 1994; 77: 2740-2747
    PubMedGoogle Scholar
  • 22 Martin DS, Levett DZH, Mythen M, Grocott MPW. Changes in skeletal muscle oxygenation during exercise measured by near-infrared spectroscopy on ascent to altitude. Critical Care 2009; 13: S7
    CrossrefPubMedGoogle Scholar
  • 23 Matzi V, Lindenmann J, Muench A, Greilberger J, Juan H, Wintersteiger R, Maier A, Smolle-Juettner FM. The impact of preoperative micronutrient supplementation in lung surgery. A prospective randomized trial of oral supplementation of combined α-ketoglutaric acid and 5-hydroxymethylfurfural. Eur J Cardiothorac Surg 2007; 32: 776-782
    CrossrefPubMedGoogle Scholar
  • 24 Mazzulli JR, Hodara R, Summer L, Ischiropoulos H. Oxidative stress and protein deposition diseases. Protein Rev 2007; 24: 123-133
    PubMedGoogle Scholar
  • 25 Mohanraj P, Merola AJ, Wright VP, Clanton TL. Antioxidants protect rat diaphragmatic muscle function under hypoxic conditions. J Appl Physiol 1998; 84: 1960-1966
    PubMedGoogle Scholar
  • 26 Ott M, Gogvadza V, Orrenius S, Zhivotovsky B. Mitochondria, oxidative stress and cell death. Apoptosis 2007; 12: 913-922
    CrossrefPubMedGoogle Scholar
  • 27 Pedersen BK, Hoffman-Goetz L. Exercise and the immune system: regulation, integration, and adaptation. Physiol Rev 2000; 2: 1055-1081
    PubMedGoogle Scholar
  • 28 Piaoux V, Mounier R, Ponsot E, Rock E, Mazura A, Dufour S, Richard R, Richalet JP, Coudert J, Fellmann N. Effects of exercise and training in hypoxia on antioxidant/pro-oxidant balance. Eur J Clin Nutr 2006; 60: 1345-1354
    CrossrefPubMedGoogle Scholar
  • 29 Reid MB. Invited review: redox modulation of skeletal muscle contraction: what we know and what we don’t. J Appl Physiol 2001; 90: 724-731
    CrossrefPubMedGoogle Scholar
  • 30 Reiter CD, Teng RJ, Beckman JS. Reaction of superoxide and nitric with peroxynitrite. J Biol Chem 2000; 275: 32460-32466
    CrossrefPubMedGoogle Scholar
  • 31 Schneid C, De Bandt JP, Cynober L, Torres E, Reach G, Darquy S. In vivo induction of insulin secretion by ornithine alpha-ketoglutarate: involvement of nitric oxide and glutamine. Metabolism 2003; 52: 344-350
    CrossrefPubMedGoogle Scholar
  • 32 Stuehr DJ. Enzymes of the L-arginine to nitric oxide pathway. J Nutr 2004; 134: S2748-S2751
    PubMedGoogle Scholar
  • 33 Subudhi AW, Jacobs KA, Hagobian TA, Fattor JA, Muza SR, Fulco CS, Cymerman A, Friedlander AL. Changes in ventilatory threshold at high altitude: effect of antioxidants. Med Sci Sports Exerc 2006; 38: 1425-1431
    CrossrefPubMedGoogle Scholar
  • 34 Wagner PD. The physiological basis of reduced VO2max in operation Everest II. High Alt Med Biol 2010; 11: 209-215
    CrossrefPubMedGoogle Scholar
  • 35 Wehrlin JP, Hallen J. Linear decrease in VO2max and performance with increasing altitude in endurance athletes. Eur J Appl Physiol 2006; 96: 404-412
    CrossrefPubMedGoogle Scholar