The protein-protein interaction network within human AF4 and AF4-MLL protein complexes

We have recently established the composition and function of the human AF4 and AF4-MLL fusion protein complex (Benedikt et al., 2011). The AF4 complex is responsible for transcriptional elongation, while the AF4-MLL complex is causing ectopic transcription, ectopic histone signatures and was shown to induce proB ALL in a mouse model. The composition of both complexes has been eludidated by using affinity-purification from human cells, nano LC-MS/MS analyses, Western blot and co-IP experiments. Functional analysis was performed by using different in vitro and in vivo assays. Here, we will present our preliminary data on the dissection of the protein-protein interaction network of complex components. This work has been done by subcloning about 35 protein-coding fragments in bait and prey vectors, and to use these vectors in combination with the yeast-2-hybrid system to unravel the AF4-N protein interactome.

This work is supported by grant Ma 1876/10–1 from the Deutsche Forschungsgemeinschaft.