Planta Med 2012; 78 - P_63
DOI: 10.1055/s-0032-1307571

Establishment and Validation of Total Quantum Statistical Moment Similarity for Radix et Rhizoma Rhei Chromatographic Fingerprints

H Sheng 1, WL Liu 1, W Wang 1, JL Shi 1, KW Deng 4, FY He 1, 2, 3
  • 1School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208 China
  • 2Property and pharmacodaynamic Key laboratory of CMM, State Administration of Traditional Chinese Medicine, Changsha 410208 China
  • 3Pharmaceutical Preparation Technology and Evaluation Laboratory of TCM, Hunan University of Chinese Medicine, Changsha, 410208 China
  • 4The First Affinity Hospital, Hunan University of Chinese Medicine, Changsha, 410007, China

A new qualitative and quantitative analytical method for the interpretation of chromatographic fingerprints for Radix et Rhizoma Rhei was elucidated and established by using the original total quantum statistical moment similarity model (TQSMS) [1,2]. The TQSMS model consists of five main parameters: total quantum statistical moment similarity (ST), total mean variability (DT), total variable probability (PT) and total variable confident probability (1-β(á)), as well as total stable confident probability (β(1-á)). The TQSMS parameters of the chromatographic fingerprints for Radix et Rhizoma Rhei from different producing locations were obtained as ST 0.94±0.04, DT 0.07±0.05, PT 0.05±0.04, 1-β (á) 0.029±0.004 and β (1-á) 0.941±0.007, respectively. ST was higher than the confident critical value of 0.803. The results displayed the similarity of the chromatographic fingerprint for Radix et Rhizoma Rhei from different places and the TQSMS parameters might be considered as characteristics for the profile of chromatographic fingerprints [3].

Fig.1: The chromatograph fingerprints of the Radix et Rhizoma Rhei from different locations of China. (S1 Gansu, S2 Jingsu, S3 Shanghai, S4 Beijing, S5 Ganjou, S6 Yunnan, S7 Sichuan, S8 Hunan, S9 Jiangxi).

Acknowledgements: Thanks Dr. Honghao Zhou, Pharmacogenetic Research Institute, Central South University, for partial support for this research. Funding was also provided by the National Natural Science Foundation of China grant, No 81073142, 81173558. References: [1] He FY, Zhou HH, et al. (2008) Yao Xue Xue Bao 43: 195–201. [2] Martinez MN (1998)J Am Vet Med Assoc 213: 974–80. [3] Wang LX, Xiao HB, et al. (2002) Journal of Medicine 37: 713–717.