Planta Med 2012; 78 - P_53
DOI: 10.1055/s-0032-1307561

Evaluation of Antioxidant Activity of Flavonoids and Phloroglucinols from Guanzhong

S Zhang 1, H Zhao 1, Q Zhang 1, Y Lin 2, Z Gao 1
  • 1School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 100102, China
  • 2School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing, 100029, China

The rhizome of Woodwardia unigemmata (Makiur.) Nakai (Blechnaceae), Lunathyrium acrostichoides (SW.) Ching (Thyriaceae), and Dryopteris crassirhizoma Nakai (Dryopteridaceae) are all used as traditional Chinese medicine, Guanzhong, [1,2]. Chinese use them for the treatment of flu, bronchitis, and verminosis [3–5]. After the chemical investigation of these three sources of Guanzhong, total flavonoids I and II were obtained from W. unigemmata and L. acrostichoides respectively, and the total phloroglucinols has been isolated from D. crassirhizoma. In this study, the antioxidant activities of flavonoids I, flavonoids II, and phloroglucinols from the above three sources of Guanzhong were evaluated in vitro by two methods including 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide anion radical scavenging ability using the chemiluminescence method. They all effectively scavenged DPPH and superoxide anion radicals and were in a dose-dependent manner. The antioxidant potential of flavonoids I, flavonoids II, and phloroglucinols in terms of IC50 values were 0.023mg/mL, 0.078mg/mL, 0.050mg/mL and 0.130mg/mL, 0.120mg/mL, 0.270mg/mL on DPPH radicals and superoxide anion radicals, respectively. The antioxidant activities were compared to a standard antioxidant, ascorbic acid. This is the first report on the antioxidant activities of these species. Acknowledgements: Thanks for the support from the innovation team of science and technology project (The Discovery and Development of Leading Compounds from TCM) of Beijing University of Chinese Medicine. References: [1] Gao Z, Wang B, et al. (2003) Zhong Yi Yao Xue Kan 21: 824–826. [2] Gao Z, Su Y, et al. (2009) Journal of Beijing University of Traditional Chinese Medicine 32: 568–570. [3] The Pharmacopoeia Commission of P.R.China. (2010) The Pharmacopoeia of P.R. China I: 310. [4] Peigen X. (2002) Xin Bian Zhong Yao Zhi II: 615–617. [5] Medical College of New Jiangsu (1977) Zhong Yao Da Ci Dian, p.1484.