Planta Med 2012; 78 - P_31
DOI: 10.1055/s-0032-1307539

Bioactive Metabolites from Cladosporium cladosporioides Against Plant Pathogens

X Wang 1, MM Radwan 1, J Gao 1, AH Tarawneh 1, DE Wedge 2, HG Cutler 3, S Cutler 1
  • 1Department of Medicinal Chemistry, University of Mississippi, University, MS-38677
  • 2US Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit, University of Mississippi, University, MS 38677
  • 3Natural Products Discovery Group, College of Pharmacy and Health Sciences, Mercer University, Atlanta, Georgia, 30341, USA

In our continuing study for bioactive constituents from fungi extracts, 40 fungi crude extracts were screened using a direct-bioautography coupled Colletotrichum bioassay. The acetone extracts of Cladosporium cladosporioides showed significant activity and was selected for further studies. Bioassay-guided fractionation of C. cladosporioides crude extracts led to the isolation of five compounds, including cladosporin (1), isocladosporin (2), 5'-hydroxyasperentin (3), cladosporin-8-methyl ether (4) and 6, 5'-diacetyl cladosporin (5). Pure compounds were further evaluated for their antifungal activity using an in vitro micro-dilution broth assay. Compound 1 possessed promising antifungal activity at 30µM with over 90% growth inhibition. Other compounds showed antifungal activities with growth inhibition between 53.9%-83.0%. The further isolation and in vivo antifungal activity studies of pure compounds are under way in order to fully evaluate the antifungal properties of C. cladosporioides.