Glycerol Metabolism Alteration in Adipocytes from n3-PUFA-Depleted Rats, an Animal Model for Metabolic Syndrome

L. Portois; Y. Zhang; J. Perret; K. Louchami; N. Gaspard; E. Hupkens; N. Bolaky; V. Delforge; R. Beauwens; W. J. Malaisse; A. Sener; Y. A. Carpentier; C. Delporte

doi:10.1055/s-0031-1297942

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2012; 44(01): 28-32
DOI: 10.1055/s-0031-1297942

Original Basic

Glycerol Metabolism Alteration in Adipocytes from n3-PUFA-Depleted Rats, an Animal Model for Metabolic Syndrome

L. Portois

¹Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium

,

Y. Zhang

²Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium

,

J. Perret

³Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium

,

K. Louchami

²Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium

,

N. Gaspard

³Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium

,

E. Hupkens

²Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium

,

N. Bolaky

³Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium

,

V. Delforge

¹Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium

,

R. Beauwens

⁴Laboratory of Cell and Molecular Physiology, Université Libre de Bruxelles, Brussels, Belgium

,

W. J. Malaisse

²Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium

,

A. Sener

²Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium

,

Y. A. Carpentier

¹Laboratory of Experimental Surgery L. Deloyers, Université Libre de Bruxelles, Brussels, Belgium

,

C. Delporte

³Laboratory of Biological Chemistry and Nutrition, Université Libre de Bruxelles, Brussels, Belgium

› Author Affiliations

Abstract

Aquaglyceroporin 7 (AQP7) is a glycerol transporter expressed in adipocytes. Its expression has been shown to be modulated in obesity. Metabolic syndrome is characterized by abdominal obesity, insulin resistance, dyslipidemia, and hypertension. An animal model displaying several features of metabolic syndrome was used to study the AQP7 expression at both mRNA and protein level and glycerol flux in adipocytes. Second generation n3-PUFA depleted female rats is a good animal model for metabolic syndrome as it displays characteristic features such as liver steatosis, visceral obesity, and insulin resistance. Our data show a reduced expression of AQP7 at the protein level in adipose tissue from n3-PUFA-depleted rats, without any changes at the mRNA levels. [U-¹⁴C]-Glycerol uptake was not modified in adipocytes from n3-PUFA-depleted animals.

Key words

aquaporins - fat cell - lipid metabolism

Full Text

References

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