Planta Med 2011; 77 - PM192
DOI: 10.1055/s-0031-1282950

Two matrix metalloproteinase inhibitors from Scrophularia striata Boiss

H Monsef Esfahani 1, R Hajiaghaee 2, A Shahverdi 3, M Khorramizadeh 4, M Amini 5
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • 2Department of Pharmacognosy & Pharmaceutics, Institute of Medicinal Plants, ACECR, Tehran, Iran
  • 3Department of Pharmaceutical Biotechnology and Pharmaceutical Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  • 4Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • 5Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Because the activation of metalloproteinases (MMPs) is a key factor in the metastatic process, compounds with the ability to inhibit MMP activity have a potential in treatment of tumor. The genus Scrophularia, consisting of about 300 Species, is one of the most important genera belonging to the Scrophulariaceae. In this study, two active substances of S. striata Boiss. were identified by using bioassay-guided fractionation and their chemical structures were deduced by nuclear magnetic resonance and mass spectrometry.

Nepitrin at lower doses showed progressive MMPs inhibitory effect with negligible cytotoxicity, whereas acteoside 1at higher doses (up to 80µg/ml) revealed most MMPs inhibitory effect preserving low cytotoxicity.

Keywords: Scrophularia striata, nepitrin, acteoside 1, wehi-164, zymoanalysis

References: Hajiaghaee R et al. (2007) Phytother Res 21: 1127–1129.