Planta Med 2011; 77 - PM82
DOI: 10.1055/s-0031-1282840

Evaluation of antinitrosative activities of selected plant polyphenols

HM Awad 1, K Mahmoud 2, HI Abd Alla 3, SA El Toumy 1
  • 1Dept. Tanning Materials and Leather Technology, National Research Centre, 12622 Dokki, Cairo, Egypt.
  • 2Dept. Pharmacognosy; National Research Centre, 12622 Dokki, Cairo, Egypt.
  • 3Dept. Chemistry of Natural Compounds, National Research Centre, 12622 Dokki, Cairo, Egypt.

The involvement of free radicals as reactive oxygen (ROS) and reactive nitrogen species (RNS), specially their increased production, appears to be a common feature to most human diseases, including cardiovascular disease, neurodegeneration and cancer [1, 2]. The treatment with antioxidant substances and other strategies leading to the reduction of oxidative and nitrosative stress may represent a therapeutic intervention that could reduce the progression of the pathological process. As such, plant polyphenolics have been suggested to play particularly important role to fight against these diseases, by affording protection towards free radical damage in cellular DNA, lipids and proteins[3–7]. Our goal was herein to investigate the scavenging capacity of some plant polyphenolic derivatives using different antinitrosative assays at different concentrations (from 0 to 300µM). In addition, the anti-proliferative activity against different human tumor cell lines was estimated using the MTT and LDH assayes. The ability of eight plant polyphenolic derivatives to react with the biologically relevant reactive nitrogen species, nitric oxide, peroxynitrite and nitrous acid were investigated indirectly by measurement of their ability to inhibit RNS-induced tyrosine nitration in vitro. All the investigated plant polyphenolic derivatives were found to be potent reactive nitrogen species scavengers and resulted in a significant inhibition of 3-nitrotyrosine (3-NT) formation in a dose-dependent manner. All the IC50s were being found at the µM level. These results indicate that these compounds may be utilized as promising sources of therapeutics.

Keywords: reactive nitrogen species (RNS), nitric oxide, peroxynitrite and nitrous acid, inhibition, 3-nitrotyrosine

Acknowledgement: This work was supported financially by the Science and Technology Development Fund (STDF), Egypt, Grant No 260.

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