Planta Med 2011; 77 - PM58
DOI: 10.1055/s-0031-1282816

Simultaneous determination of some phenolic compounds and antioxidant activity of Inula viscosa (L.) Aiton

A Gökbulut 1, E Şarer 1, B Satılmış 2, K Batçıoğlu 2
  • 1Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100, Tandoğan, Ankara, Turkey
  • 2Department of Biochemistry, Faculty of Pharmacy, İnönü University, 44280, Malatya, Turkey

Inula species are widespread in the world and used traditionally for ages by different cultures due to their various biological activities. The members of this genus contain terpenic compounds, especially sesquiterpene lactones, flavonoids, glycolipids and anthranilic acid derivatives (1,3). I. viscosa, suffrutescent and rank-smelling herb up to 1–2m, is widespread in Mediterranean area (4). In this study, antioxidant activity of freeze dried water, methanol and ethyl acetate extracts of flower, leaf and radix of I. viscosa were evaluated via DPPH and ABTS methods. All the extracts showed antioxidant activity in different concentrations. Water extract of I. viscosa flower expressed strong antioxidant activity with lower IC50 values with both methods compared with the other extracts. Ethyl acetate extracts of the investigated parts of the plant showed less antioxidant activity compared with the water and methanol extracts. It's obvious that phenolics are responsible for the antioxidant potential of the plants. For this reason, phenolic compounds such as chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, luteolin and kaempherol were analyzed qualitatively and quantitatively in the flower, leaf and radix methanol extracts of I. viscosa by RP-HPLC. Chlorogenic and caffeic acids were found in all the investigated parts of the plant. Only myricetin was absent in the flower extract and chlorogenic acid was found in significant amount in radix extract. While myricetin was not determined in the plant, kaempherol was found only in the flower extract. Therefore, most of the investigated phenolics could be responsible for the potent antioxidant activity of I. viscosa

Keywords: Inula, Antioxidant activity, DPPH, ABTS, RP-HPLC

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2. Danino O et al. (2009) Food Res Int 42: 1273–1280.

3. Shan J-J et al. (2006) Biol Pharm Bull 29(3): 455–459.

4. Davis PH (1982) Flora of Turkey and The East Aegean Islands, Edinburgh University Press, Edinburgh.