Planta Med 2011; 77 - PM25
DOI: 10.1055/s-0031-1282783

Screening on cytotoxicity, antioxidant and antimicrobial of stem bark from Malaysian Vatica odorata and Vatica bella (Dipterocarpaceae)

WZ Wan Mohd Zain 1, N Ahmat 1, J Latip 2, SZ Mat So'at 3, S Daud 4
  • 1Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia
  • 2School of Chemistry and Food Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
  • 3Kulliyah of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
  • 4Faculty of Applied Sciences, Universiti Teknologi MARA, 26400 Jengka, Pahang, Malaysia

Dipterocarpaceae have proven to be rich sources of variety biological activities (Zain et al.2010). In our continuing investigation on this family we wish to report the screening on three biological activities: cytotoxicity, antioxidant and antimicrobial of methanol and acetone extract from Vatica odorata (Griff.) Symington and Vatica bella Slooten. The antioxidant activity were evaluated by 1,1-diphenyl-2-picrylhydrazil (DPPH), Total Phenolic Content (TPC), Ferric thiocyanate method (FTC) and Thiobarbituric acid (TBA) method. The cytotoxicity activities were screened against Chang and HepG2 cells line (Mackeen et al. 1997). Meanwhile antimicrobial activity was conducted against six types of bacteria (Escherichia Coli, Stapylococcus aureus, Bacillus subtilis, Salmonella typhi, Pseumodonas aerugonisa and Klebsiella pneumonia) and four dermatophyte fungal species (Trichophyton mentagrophytes, Trichophyton tonsurans, Microsporum gypseum and Candida glabrata) by disc diffusion method (Barry et al.1979). The results indicated that acetone extract of Vatica bella displayed moderate activity against Chang's liver cell and HepG2 cell with IC50 values of 14±0.50µg/mL and 20.5±2.68µg/mL respectively. Both the Vatica extract displayed total phenolic content with range of 331.54–482.31mg/g GAE and are weak DPPH scavenger as compared to standard with the range between 35.60–66.2%. In FTC and TBA test, the Vatica extracts exhibited antioxidant potential with percent inhibition between the ranges 26.60–88.36%. The antimicrobial screening showed that both the crude extract inhibited moderately S.aureus except for acetone extract of Vatica bella. Meanwhile methanol extract of Vatica bella gave the active result where it inhibited moderately against Trichophyton mentagrophyte, Trichophyton tonsurans and Microsporum gypseum.

Keywords: Vatica bella, Vatica odorata, Dipterocarpaceae, oligostilbenoid, cytotoxicity, antimicrobial, antioxidant

Acknowledgement: We wish to thank to Ministry of Higher Education Malaysia for financial support via FRGS grant (011000070006) and UiTM for all the support

References: 1-Zain WZWM, Ahmat N, Nawi L.& Jusoh, K (2010) World Applied Science Journal 8(9):1050–1055.

2-Mackeen MM et al. (1997) Int J Pharmacognosy 35: 174–178

3- Barry AL et al. (1979). Journal of Clinical Microbiology 10: 885–889.