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DOI: 10.1055/s-0031-1282775
In vivo evaluation of an herbal remedy for antimalarial activity
The antimalarial activity of an herbal remedy (HR) formulated, based on ethnomedical claims followed by observational experiences, was investigated in Plasmodium berghei NK 65- infected mice. The decoction of the HR was prepared, concentrated in vacuo and freeze-dried. Evaluation of the antimalarial activity involved the use of early malarial (4 -day test) and established infection models [1, 2]. The HR was tested at 15–240mg/kg while the positive control, amodiaquine (AQ) was tested at 1.25–10mg/kg. For the established infection test, the HR was tested at 60–240mg/kg with AQ (10mg/kg) as positive control. Distilled water was used as negative control in both test. The HR and AQ gave ED50 of 40 and 3.8mg/kg respectively, while for the established infection test, the highest dose of 240mg/kg gave 54.45% clearance on day 5. The HR showed higher suppressive than curative activity.
Keywords: Herbal remedy, Plasmodium berghei, Amodiaquine
Acknowledgement: Prof. G. A. Ademowo, Department of Pharmacology and Therapeutics, University of Ibadan, Ibadan, Nigeria for access to the Plasmodium berghei NK 65 parasite
References: 1. Peters W (1965) Exp Parasitol 17: 80–87
2. Ryley J, Peters W (1970) Am J Trop Med Parasitol 84: 209–211