Planta Med 2011; 77 - PL88
DOI: 10.1055/s-0031-1282737

Quantitative analysis of rosmarinic acid in Rosmarinus officinalis growing in Turkey by LC-MS/MS

A Altintas 1, F Göger 1, HG Duymuş 1, N Kırımer 1, KHC Başer 1, 2
  • 1Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, 26470 Eskişehir, Turkey
  • 2King Saud University, College of Science, Botany and Microbiology Department, P.O. BOX 2455– Riyadh 11451- Saudi Arabia

Rosmarinic acid is naturally occuring polyphenolic compound mostly found in Lamiaceae family herbs such as rosemary, perilla, oregano, sweet basil. It exhibits various biological activity like antioxidant, antiviral, antibacterial and antiinflammatory (1,2).

LC-MS/MS has an important role in the studies of identification and characterization of natural compounds, drug metabolism, discovery of new drug candidates because of its both sensivity and specificity.

In this present study, we aimed to quantify of rosmarinic acid in methanol extract of Rosmarinus officinalis leaves collected from İzmir by using a rapid LC-MS/MS method. Therefore we used turbo spray ionization for LC-ESI-MS method in negative mode. Rosmarinic acid was characterized by its MS/MS spectrum and LC retention time. The assay performed in different concentrations of rosmarinic acid as standart solutions. The diagnostic fragmentations 358.9/160.9 and 358.9/197.0 of rosmarinic acid were used for the quantification. As a result, 0.31g rosmarinic acid measured in 100g methanol extract of rosemary leaves.

References: 1. Petersen M, Simmonds MSJ (2003) Phytochem 62:121–125.

2. Park SU, Uddin MR, Xu H, Kim YK, Le, S (2008) African J Biotechn 7(25): 4959–4965.