Bioactive compounds from Phyllanthus atropurpureus Bojer cultivated in Egypt

From the ethyl acetate soluble fraction of the Phyllanthus atropurpureus Bojer, six compounds were isolated and identified based on spectral data (IR, UV, Mass (FAB, EI), ¹H-NMR and ¹³C-NMR). Four compounds are isolated for the first time from the plant, the structures were established as di [3, 4, 5- trihydroxy- phenyl] ether, 5, 6, 8, 4'-tetrahydroxy isoflavone, Robustaside A, 6'- (4“- hydroxy cinnamoyl) arbutin, and 6'- (3“, 4“- dihydroxy cinnamoyl) arbutin. The other two isolated compounds are Demethoxysudachitin, and quercetin-7- O-glucoside.

Concerning the biological studies, the results revealed that the total extracts can be considered an efficient antimicrobial, when ethyl acetate fraction of leaves shows significant gram -ve antibacterial activity (E. coli) than the other tested materials as well as Ampicillin. All tested materials show significant gram +ve antibacterial activity (S. aureus) compared to the effect of Gentamycin. Also robustaside A was found to produce a specific strong anti-tumor activity against hepatocellular carcinoma.