The effect of Salvia virgata on GSH-Px Activities of HepG2 cells.

Turkey is an important country for Salvia species. The flora of Turkey includes 88 species of the genus Salvia. Salvia virgata Jacq. which has shown to be extremely rich with the phenolic compounds that allows this species to be an important member of antioxidant plants. This study was performed to investigate the effect of different Salvia extracts on GSH-Px activities of HepG2 hepatocarcinoma cells.

The 70% methanol and water extracts were prepared from the aerial parts of S. virgata collected from Bursa, Turkey. Gallic acid and rosmarinic acid were used as positive controls. The cells at a number of 2×10⁵ cells per well were incubated for 24h with the extracts and the positive controls under %5 CO₂ at 37°C. The GSH-Px activities of the cells were than analysed spectrophotometrically via a multifunctional microplate reader.

Phenolics rich extract of aq. methanol has enhanced the GSH-Px activity more than water extract where their effect was just in between the rosmarinic acid and gallic acid positive controls.

These results reveal that both extracts mostly the phenolics rich extract of aq. methanol supports the antioxidant activity in the hepatocarcinoma cell line and these results confirm that it can further effect the glutathione reserves of these cells. This peliminary results needs to be further investigated over the GSSG, GSH and total gluathione and selenium levels.

Keywords: Salvia Virgata, HepG2, GSH-Px, antioxidant

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