This study investigated the burn healing efficiency of Black seeds (Nigella sativa L.) oil on the second degree burn wound models in rats. Many of pharmacological activities
of Nigella sativa seeds such as anti-inflammatory and antioxidant were due to unsaturated fatty acids
and essential oil [1,2,3]. In this study the hexanic extract of seeds was topically
applied to evaluate the healing activity of seeds oil. Animals were randomly divided
into three groups of six for each group. Burn wounds were created on dorsal part of
shaved rats by a soldering iron with a flat contact surface (diameter 1.5cm) on top
(100°C for 10 seconds). Silver sulfadiazine (SSD) was used as an antiseptic standard
drug. Wound healing was evaluated by the rate of contraction and histological characteristics
in treated and untreated groups. On day 12, the extract-treated animals showed 81.20%
decreasing in the wound district and were significantly (P<0.05) more than control
group 63.31%. Histological study showed fully grown regenerated epidermis on day 12
in treated animals. The results of this study suggest that burn wound healing potential
of seeds may be due to anti-inflammatory, antioxidant and antimicrobial activities
of main compounds oil.
