Effect of the extracts of Piper cumanense and Piper eriopodon in the behavior of genes involved in oxidation process of the skin

Nowadays the role of the genes is a tool of study to achieve an accurate form to avoid in this particular case the skin degeneration. The propose of this study was the method design to evaluate the behavior of certain genes as MMP1, MMP7, MMP9, MMP11, MMP12, COL3A1, COL1A2 and ELASTIN, (which are involved in degradation process caused by the oxidation), in in vitro assays with Human Dermal Fibroblast through the use of Real Time RTPCR, as well as, the evaluation of butanolic extracts of Piper cumanense Kunth and Piper eriopodon C.DC. over these cells to determine the effect to above genes; said extracts were rich in flavonoids. The concentration used for Piper cumanense was 18µg/mL and for Piper eriopodon was 6µ/mL. The Real Time RTPCR study showed an important relative change in the expression of MMP1 (0,00005), MMP2 (0,00020), MMP3 (0,18956), MMP9 (3,64782), MMP12 (5,34601), COL1A2 (0,21653), COL3A1 (0,01603), ELASTIN (0,03221) genes of the cells trated with Piper cumanense extract; and for the Piper eriopodon extract, the important relative change was, MMP1 (0,00003), MMP3 (04,56702), MMP9 (0,23369), MMP12 (0,54334), ELASTIN (10,69215), These results suggest that the extracts could have an influence over the oxidation process in which these genes are involved, and the use of the Real Time RTPCR methodology allows the elucidation of this influence.

Acknowledgement: Dr. Fabio Aristizábal, professor, Department of Pharmacy, National University

Colombia and Linamaría Escobar.