Planta Med 2011; 77 - PG94
DOI: 10.1055/s-0031-1282578

New N-alkylamides from Anacyclus pyrethrum

J Boonen 1, V Sharma 2, V Dixit 2, B De Spiegeleer 1
  • 1Drug Quality and Registration (DruQuaR) group, Department of Pharmaceutical analysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium
  • 2Department of Pharmaceutical Sciences, Dr. H.S. Gour University, Sagar, India

The roots of Anacyclus pyrethrum DC (Asteraceae) are frequently used in traditional medicine e.g. as aphrodisiac [1]. Depending on the extraction method and solvent, different yields of N-alkylamide constituents can be found, possibly resulting in alterations in biological effects and toxicity. Therefore, analytical profiling of the bio-active N-alkylamides in these plant preparations is an inevitable quality parameter, with liquid chromatography-electrospray mass spectrometry (HPLC/ESI-MS) as recommended technique for comprehensive analysis of alkylamides in plant extracts [2–4].

An N-alkylamide profiling from an ethanolic Anacyclus pyrethrum root extract was performed using a gradient reversed phase HPLC/ESI-MS method on an embedded polar column. MS1 and MS2 fragmentation data were used for identification purposes, while UV was used for quantification. Thirteen N-alkylamides (five N-isobutylamides, three N-methyl isobutylamides, four tyramides and one 2-phenylethylamide) were detected. Five of them are novel compounds, which have never been identified in Nature.

Acknowledgement: Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) (no. 091257) and the All India Council for Technical Education, New Delhi, India.

References: 1. Sharma V, Thakur M, Chauhan N, Dixit V (2010) Planta Med 76: 1214–1214.

2. Sharma V, Boonen J, Chauhan N, Thakur M, De Spiegeleer B, Dixit V (2011) Phytomedicine, In press.

3. Kartal M, Kan, Gulpinar AR (2007) Planta Med 73: 253.

4. Boonen J et al. (2010)J Pharmaceut Biomed 53: 243–249.