New N-alkylamides from Anacyclus pyrethrum
The roots of Anacyclus pyrethrum DC (Asteraceae) are frequently used in traditional medicine e.g. as aphrodisiac . Depending on the extraction method and solvent, different yields of N-alkylamide constituents can be found, possibly resulting in alterations in biological effects and toxicity. Therefore, analytical profiling of the bio-active N-alkylamides in these plant preparations is an inevitable quality parameter, with liquid chromatography-electrospray mass spectrometry (HPLC/ESI-MS) as recommended technique for comprehensive analysis of alkylamides in plant extracts [2–4].
An N-alkylamide profiling from an ethanolic Anacyclus pyrethrum root extract was performed using a gradient reversed phase HPLC/ESI-MS method on an embedded polar column. MS1 and MS2 fragmentation data were used for identification purposes, while UV was used for quantification. Thirteen N-alkylamides (five N-isobutylamides, three N-methyl isobutylamides, four tyramides and one 2-phenylethylamide) were detected. Five of them are novel compounds, which have never been identified in Nature.
Acknowledgement: Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT-Vlaanderen) (no. 091257) and the All India Council for Technical Education, New Delhi, India.
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