The Constituents from the Stem of Clausena lansium and their Bioactivities
Two new glycosides, clausenosides A (1) and B (2) and three new carbazole alkaloids, clausenaline A (3), claulamine A (4) and claulamine B (5), together with fifty known compounds were isolated from the stem of Clausena lansium Skeels. Their structures were determined by spectroscopic methods, including CD spectroscopy and 1D and 2D NMR spectra. Compound 4 has an 1-oxygenated carbazole framework with a rare 2,3-lactone ring. Compound 5 represents the first acetal carbazole alkaloid, of which the absolute configuration was determined by Mosher's method. The cytotoxicity of compounds 3-6 against a limited panel of cancer cell lines and the anti-inflammatory activity of 8-27 were evaluated.
Acknowledgement: The authors are grateful for financial support from the National Science council of Republic of China awarded to T.-S. W. Thanks are also given to Associate Prof. Vu Xuan Phuong (Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology). This study was supported in part by Taiwan Department of Health Clinical Trial and Research Center of Excellence (DOH 100-TD-B-111–004).