Isolation, Purification and Structure Elucidation of Diterpenoids from the Roots of Salvia chorassanica Bunge
Salvia contains around 900 species worldwide, mainly in central Asia. The genus has 58 species and one hybrid in Iran, in which 17 of them are endemic to the country . Salvia chorassanica Bunge is one of the Iranian endemic species of Salvia that only grows in Iran that belonges to the Lamiaceae family. There is not any reported literature on S. chorassanica so the present project set to start search for finding various diterpenoids from this plant. Air- dried and powdered roots of S. chorassanica were extracted with EtoAc (3×3L), for about 24h at ambient temperature. After filtration, the combined extracts were consentrated yielding 20g of total extract. two diterpenoids, Taxodione (1)  and Ferruginol (2) , were isolated by means of chromatographic methods mainly column chromatography checked by TLC, and purified by preparative RP-HPLC. The structures of compounds 1–2 were determined on the basis of spectroscopic data [4,5] using 1H NMR, 13C NMR, DEPT-135, HMBC, HSQC, COSY and NOESY experiments. In conclusion, based on the result obtained from our study S. chorassanica can be considered a rich source of different abeitane diterpenoids. This is the first report of compounds isolated from this plant.
References: 1. Emami A et al. (2008) de I'Universite d'Iran des Sciences Medicales pp. 362–391.
2. Kupchan SM et al. (1968) Journal of the American Chemical Society 90: 5923–5924.
3. Son Kh et al. (2005) Bioorganic & Medicinal Chemistry Letters 15: 2019–21.
4. Marcos IS et al. (2010) Tetrahedron 66: 7773–7780.
5. Tezuka Y et al. (1998) Pharmaceutical Society of Japan 46: 107–112.