Planta Med 2011; 77 - PG64
DOI: 10.1055/s-0031-1282548

Constituents of Verbascum reeseanum

BJ Djimtombaye 1, T Karayıldırım 1, M Masullo 2, S Piacente 2, D Gülcemal 1, SG Şenol 3
  • 1Ege University, Faculty of Science, Department of Chemistry, Bornova, 35100, İzmir, Turkey
  • 2Salerno University, Department of Pharmaceutical Sciences, 84084 Fisciano (Salerno), Italy
  • 3Department of Biology, Faculty of Science, Ege University, Bornova, 35100 İzmir, Turkey

The genus Verbascum (Scrophulariaceae) is represented by 228 species, of which 185 are endemic in the flora of Turkey and East Aegean Islands [1]. Verbascum species contain biologically active compounds, such as flavonoids, phenylethanoid and neolignan glycosides, saponins, and iridoid and monoterpene glycosides [2]. The leaves and flowers of Verbascum are reported to have expectorant, mucolytic and demulcent properties, and are used to treat respiratory disorders such as bronchitis, dry coughs, tuberculosis and asthma in traditional Turkish medicine [3].

In this study, dried and grinded plant material (Verbascum reeseanum Hub.-Mor. – whole plant) was extracted with MeOH, and then treated with n-hexane. The n-butanol fraction from the methanol extract was submitted to several preparative chromatographic methods such as VLC, normal phase silica gel CC and reversed phase RP-18. The structures of the known compounds were determined as ajugol, lawsoniaside B, ajugoside, coniferin and sinuatol, by a combination of one- and two-dimensional NMR techniques, and mass spectrometry.

References: 1. Davis PH (1978) Flora of Turkey and East Aegean Islands. University Press. Edinburgh

2. Tatli II, Akdemir ZŞ (2004) Fabad J Pharm Sci 29: 93–107.

3. Tatli II, Akdemir ZŞ (2006) Fabad J Pharm Sci 31: 85–96.