Isolation of antocyanins with identified qualitative-quantitative properties
Technology of natural substances isolation is substantial element affecting on the quality of natural preparations. The isolation of the plant substances from a raw material is carried out by distillation and extractions with different solvents. The methods used for anthocyanin separation due to high temperatures, etc. destroy their structures and decrease their therapeutic effects. The present study is aimed to establish optimal procedures for anthocyanins after their extraction to secure the stability of substances by lyophilisation. The freeze drying technology seems to be an appropriate method to keep the structure and qualitative-quantitative properties of these natural components. The focus of lyophilisation technology is vacuum sublimation of ice crystals, i.e. phase transition from solid to gas state, which is created during freezing the water solution. The process is carried out in three phases: freezing, primary drying and secondary drying. LC/MS/IT/TOF equipment is used for the determination of anthocyanin qualitative-quantitative characteristics before and after lyophilisation. The biological and antimicrobial properties of these natural components are tested as well. The freeze drying technology used is established by Mediproduct Company in Lipany, the East Slovakia.
Keywords: Hydrolyzation, lyophilisation, plant material, secondary metabolites
Acknowledgement: The participation is supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic, the project: 00162–0001 (MS SR-3634/2010–11).