Genome wide expression analysis of the effect of wwcsw, a traditional Korean herbal formula, on rat intracerebral hemorrhage
Woo-whang-chong-shim-won (WWCSW) is a traditional Korean herbal formula which is commonly used for treating patients with hypertension, arteriosclerosis, coma and stroke in China and Korea. WWCSW is composed of various kinds of chemical components, it would be difficult to isolate major components having pharmaceutical effect. Therefore, high throughput screening systems such as microarray analysis is essential process to elucidate the molecular effects of herbal extract on animal disease model. In this experiment, we measured the effect of WWCSW on ICH in rat using microarray technology. ICH was induced by injection of collagenase type IV, and total RNA was isolated. Hierarchical clustering was implemented using CLUSTER and TREEVIEW program, and for Ontology analysis, GOSTAT program was applied in which p-value was calculated by Chi square or Fisher's exact test based on the total array element. WWCSW-treatment restored the gene expression altered by ICH-induction in brain to the levels of 76.0% and 70.1% for up- and down-regulated gene, respectively. Co-regulated genes by ICH model of rat could be used as molecular targets for therapeutic effect of drug including WWCSW.
References: 1. Choi EW et al. (2000) Journal of Applied Pharmacology 8: 255–61.
2. Kim BY et al. (2009) Korean J Orient Int Med 30(3): 594–60.
3. Andrew IS et al. (1979) Progress in Cardiovascular Diseases 22(1): 31–52.