Planta Med 2011; 77 - PF34
DOI: 10.1055/s-0031-1282422

Total phenolics content of the ethyl acetate extract of Salvia tomentosa

M Onay 1, N Coruh 2, F Celep 3, M Doğan 3
  • 1Middle East Technical University, Department of Biochemistry, Ankara, 06800, Turkey
  • 2Middle East Technical University, Department of Chemistry, Ankara, 06800, Turkey
  • 3Middle East Technical University, Department of Biology, Ankara, 06800, Turkey

Salvia generally including flavonoids is one of the largest genera in Lamiaceae family and this genus has 900 species. It is composed of 88 species in Turkey and mostly benefited in the treatment of skin infections, colds, stomach ache, headache and tuberculosis. In the present study, aerial part of Salvia tomentosa Mill. was used and extraction procedure was applied to its ethyl acetate extract. Yield was obtained as percentage (%). In addition, it was investigated for its total phenol content (TPC) and performed by Singleton and Rossi method with a few modifications. Gallic acid was used as standard and results were expressed as micrograms of total phenolics including of extract as gallic acid equivalents (GAE). In conclusion, its TPC was found as 103.75±4.32 GAE µg/mg of extracts. Each datum was calculated as a average of duplicate measurements acquired from at least three separate experiment sets. According to this result, when its total phenol content was compared with other Salvia species, S. tomentosa showed higher TPC than that of other Salvia species.

References: 1. Kelen M, Tepe B (2008) Bioresource Technology 99: 4096–4104.

2. Kivrak I, Dur ME, Öztürk M, Mercan N, Harmandar M, Topçu G (2009) Food Chemistry116: 470–479.

3. Singleton V L, & Rossi J A (1965) American Journal of Enology Viticulture 16:144–158.