Planta Med 2011; 77 - PE16
DOI: 10.1055/s-0031-1282347

Essential oil variability and trichomes morphology from Lavandula pedunculata (Mill.) Cav. grown at Mata Experimental do Escaroupim (Portugal)

MD Feijão 1, G Teixeira 2, T Vasconcelos 3, L Rodrigues 3, AI Correia 4, J Sanches 5, LG Pedro 6, JG Barroso 6, AC Figueiredo 6
  • 1Universidade de Lisboa, Faculdade de Ciências de Lisboa, Departamento de Biologia Vegetal, C2, Campo Grande, 1749 016 Lisboa, Portugal
  • 2Universidade de Lisboa, Faculdade de Farmácia de Lisboa, Centro de Biologia Ambiental, Avenida Prof. Gama Pinto, 1649–003, Lisboa, Portugal
  • 3Universidade Técnica de Lisboa, Instituto Superior de Agronomia, Centro de Botânica Aplicada à Agricultura, Tapada da Ajuda, 1349–017, Lisboa, Portugal
  • 4Universidade de Lisboa, Faculdade de Ciências de Lisboa, Centro de Biologia Ambiental, C2, Campo Grande, 1749–016 Lisboa, Portugal
  • 5Autoridade Florestal Nacional, Direcção Regional de Florestas de Lisboa e Vale do Tejo, 2001–901 Santarém, Portugal
  • 6Universidade de Lisboa, Faculdade de Ciências de Lisboa, Departamento de Biologia Vegetal, Instituto de Biotecnologia e Bioengenharia, Centro Biotecnologia Vegetal, C2, Campo Grande, 1749–016 Lisboa, Portugal

The Experimental Forest of Escaroupim [Mata Experimental do Escaroupim (MEE), Salvaterra de Magos, Portugal], is a protected forest area with over 175 years and under the Total Forestry Regime since 1901. Lavandula pedunculata (Mill.) Cav. is an aromatic shrub common in the Iberian Peninsula [1], and frequent in the understory of the MEE Pinus, Quercus, Ulmus and Eucalyptus spp. forests. In the present work, the essential oils and trichomes morphology from flowering aerial parts of two populations of L. pedunculata collected in two years were evaluated. The essential oils were isolated by hydrodistillation, and analyzed by GC and GC-MS [2]. The indumentum of L. pedunculata field grown plants was studied by LM and SEM, according to [3]. L. pedunculata essential oils were obtained in an average yield of 2% (v/w). Thirty six components were identified, representing 97–99% of the total essential oils, which were dominated by fenchone (62–70%) and 1,8-cineole (6–28%). cis-Verbenol (traces-5%), camphor (1–5%) and limonene (traces-4%) were also relatively abundant. Previous studies also showed essential oils fenchone-, 1,8-cineole- and camphor-rich [2,4,5]. L. pedunculata showed a morphologically complex indumentum of i) non-glandular uni- and bicellular unbranched trichomes and multi-cellular branched trichomes of the stellate type; ii) peltate and capitate trichomes, the last with three different morphological types; iii) multi-cellular branched stellate type with only glandular arms and iv) multi-cellular branched stellate type with both glandular and non-glandular arms. These results are in agreement with a previous study on the indumentum of L. pedunculata field- and in vitro-grown plants [5].

Acknowledgement: Telmo Nunes, Paula Paes, A. Sofia Borges, Prof. Ana Monteiro.

References: 1. Morales R (2010) Flora Iberica, Lavandula. Vol. XII. Real Jardín Botánico. CSIC. Madrid

2. Matos F et al. (2009)J Essent Oil Res 21: 327–336.

3. Antunes T, Sevinate-Pinto I (1991) Flora 185: 65–70.

4. Zuzarte M et al. (2009) Chem Biodivers 6: 1283–1292.

5. Zuzarte, M et al (2010) Ind Crops Prod 32: 580–587.