Essential oils composition and antioxidant properties of three Thymus species

H Amiri; H Lari Yazdi; M Dehshiri; D Eghbali; A Mohammadi; A Zarei

doi:10.1055/s-0031-1282334

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Planta Med 2011; 77 - PE3
DOI: 10.1055/s-0031-1282334

Essential oils composition and antioxidant properties of three Thymus species

H Amiri ¹, H Lari Yazdi ², M Dehshiri ², D Eghbali ², A Mohammadi ¹, A Zarei ²

¹Department of Biology, Lorestan University, Khoramabad, Iran.
²Department of Biology, Isamic Azad University, Branch of Broujerd, Broujerd, Iran.

Congress Abstract

The essential oils of three wild-growing Thymus species (Thymus kotschyanus Boiss. & Hohen., Thymus eriocalyx (Ronniger) Jalas. and Thymus daenensis Celak subsp lancifolius (Celak) Jalas. collected from west of Iran during the flowering stage, were obtained by hydrodistillation and analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS). Under the optimum extraction and analysis conditions, 44, 38 and 38 constituents (mainly monoterpenes) were identified in T. kotschyanus, T. eriocalyx and T. daenensis subsp. lancifolius which represented 89.9%, 99.7% and 95.8% of the oils, respectively. The main constituents were thymol (16.4–42.6%), carvacrol (7.6–52.3%) and γ-terpinene (3.0–11.4%). Antioxidant activity was employed by two complementary test systems namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene/linoleic acid systems. Antioxidant activity of polar sub-fraction of T. daenensis subsp lancifolius was found to be higher than those of the others in DPPH assay while non-polar sub-fraction of T. eriocalyx has most antioxidant activity in β-carotene/linoleic acid test (19.1±0.1µg/ml and 96.1±0.8% inhibition rate, respectively).