Planta Med 2011; 77 - PB50
DOI: 10.1055/s-0031-1282304

In vitro study of callus induction and regeneration in Iranian Cichorium intybus

A Zebarjadi 1, D Kahrizi 1, S Yazdani 1
  • 1Dept. of Agronomy and Plant Breeding, Faculty of Agriculture, Dept. of Biotechnology for Drought Tolerance Research, Razi University, Kermanshah, Iran

Cichorium intybus L. belongs to the Asteraceae family and is one of the medicinally important plant that contains some useful secondary metabolites such as bitter sesquiterpene lactones, coumarins and flavonoids. This plant is conventionally propagated through seeds. In this research, in vitro culture of Iranian Cichorium intybus was studied, thus an experiment was laid out as a completely randomized design (CRD) in a factorial arrangement with there replications that investigated factors were different concentration of plant growth regulators (NAA and BAP) and explants (hypocotyls and cotyledon). The results indicated that significant and non-significant differences among levels of explants and other factors for callus induction and plant regeneration, as we observed the highest percentage of callus formation from MS medium supplemented with 1.5mg/l NAA and 2mg/l BAP. Mean comparisons shown that the best explant was cotyledon versus hypocotyls for the traits and maxium of regeneration recorded for MS medium with 0.4mg/l NAA and 5mg/l BAP.